Next-Generation Sequencing (NGS) is a powerful technology that enables the analysis of genomic information at an unprecedented level of detail. The process of NGS typically involves the preparation of DNA or RNA samples, which are then sequenced using a high-throughput sequencing instrument.
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NGS sample preparation involves several key steps, which may vary depending on the specific application and the type of sample being analyzed. The general workflow for NGS sample preparation includes the following steps:
- Sample collection and extraction: The first step in NGS sample preparation is to collect the sample of interest, such as blood, tissue, or cells, and extract the DNA or RNA from the sample. This step may involve several methods, such as mechanical disruption, chemical lysis, or enzymatic digestion, depending on the sample type.
- Library preparation: After extracting the DNA or RNA, the next step is to prepare a sequencing library. This involves fragmenting the DNA or RNA into smaller pieces and attaching adapters to the ends of the fragments to enable sequencing on the NGS instrument. Different library preparation methods are available, depending on the application and the sample type.
- Quality control: Before sequencing, it is important to assess the quality and quantity of the library to ensure that it is suitable for sequencing. Quality control can be performed using several methods, such as gel electrophoresis, fluorometric assays, or qPCR.
- Sequencing: Once the library has passed quality control, it can be sequenced on an NGS instrument. The sequencing process generates large amounts of data, which can be processed and analyzed using various bioinformatics tools.
Overall, NGS sample preparation is a complex process that requires careful attention to detail to ensure the accuracy and reliability of the sequencing data. The specific steps involved may vary depending on the application and the sample type, and it is important to select the appropriate library preparation method and quality control measures for the specific experiment.
Next-generation sequencing (NGS) market is experiencing growth, due to factors like development of the products, which validate the initiation of high-quality gene libraries and the sequencing yields.
NGS has transformed the genomic research studies. NGS is an innovative technology that has accredited to sequence the whole human genome in a single day. NGS ย has high sequencing speed and enhanced extensibility, which result in positive applications for research and development. With advancements made in the sequencing technologies, the protocols for sample preparation are being changed. Pre-sequencing methods like library preparation and target enrichment are resulting in formation of correct sequencing and high-quality data. Constant innovations made in the field of NGS sample preparation has resulted in the standard sample preparation protocols boosting the growth of NGS sample preparation market. NGS finds its applications in treatment of genetic disorders and clinical diagnostics.
The NGS sample preparation market is segmented based on several factors, including the type of product, application, end-user, and region. Some of the key segments in the NGS sample preparation market include:
- By product type: The NGS sample preparation market is segmented based on the type of product, such as library preparation kits, reagents, and instruments.
- By application: The market is segmented based on the applications of NGS, such as oncology, infectious diseases, reproductive health, and genetic disorders.
- By end-user: The market is segmented based on the end-users of NGS sample preparation products, such as academic and research institutions, biotechnology and pharmaceutical companies, and clinical diagnostic laboratories.
- By region: The market is segmented based on geographic regions, such as North America, Europe, Asia-Pacific, and Rest of the World.
These segments provide a comprehensive understanding of the NGS sample preparation market, and help stakeholders to identify the key growth opportunities and challenges in the market. They also enable companies to develop targeted marketing strategies and tailor their product offerings to meet the specific needs of different customer segments.
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Top impacting factors
- Increase in the demand for personalized treatment is boosting the growth of NGS sample preparation market. Also, surge in expenditure for healthcare and advancements made in healthcare technology are the factors boosting the growth of NGS sample preparation market.
- Use of NGS in the diagnosis of diseases like cancer, diabetes, and treatment of other diseases is leading the market growth. Also, rise in the geriatric population is responsible for the market growth.
- Increase in the creation of huge data has led to problems like tracking and data management, which has been solved with the help of digital solutions like NGS samples.
- Automation made in NGS method has propelled the growth of NGS sample preparation market.
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Thermo Fisher Scientific, Agilent Technologies, Beckman Coulter (a subsidiary of Danaher), Becton Dickinson, NuGEN Technologies, Illumina Inc, QIAGEN, Promega Corporation, New England Biolabs, Tecan Trading AG
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